Be mindful even though preparing the sample and pipetting it into a cuvette or onto a microvolume platform. The sample really should be homogeneous.To produce a calibration curve, the value for the absorbances of each and every of your spectral curves at the best absorbing wavelength, is plotted in the graph similar to that in Figure (PageIndex 6 )
Be cautious while making ready the sample and pipetting it right into a cuvette or onto a microvolume platform. The sample need to be homogeneous.Detection of useful teams: U.V. spectroscopy is used to detect the existence and absence of chromophores. The absence of a band at a selected wavelength suggests the absence of a certain team In this part
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